DNA Sequencing : Laboratory Techniques in Biochemistry and Molecular Biology

John Hindley,"DNA Sequencing : Laboratory Techniques in Biochemistry and Molecular Biology"


Elsevier
1985-05-05
ISBN: 0444804978
384 pages
PDF
13.64 mb



This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam-Gilbert chemical procedure; Computer methods for DNA sequencers; Appendices including contractions and special terms, cloning vectors, commercially available restriction endonucleases, and autoradiography.

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