Molecular Cloning: A Laboratory Manual by Joseph Sambrook, David W. Russell




* Number Of Pages: 999


* Publication Date: 2001-01-15

* ISBN-10 / ASIN: 0879695773

* ISBN-13 / EAN: 9780879695774

* Binding: Paperback





Contents:

Chapter 1. Plasmids and Their Usefulness in Molecular Cloning

Chapter 2. Bacteriophage lambda and Its Vectors

Chapter 3. Working with Bacteriophage M13 Vectors

Chapter 4. Working with High-capacity Vectors

Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis

Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA

Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells

Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction

Chapter 9. Preparation of Radiolabeled DNA and RNA Probes

Chapter 10. Working with Synthetic Oligonucleotide Probes

Chapter 11. Preparation of cDNA Libraries and Gene Identification

Chapter 12. DNA Sequencing

Chapter 13. Mutagenesis

Chapter 14. Screening Expression Libraries

Chapter 15. Expression of Cloned Genes in Escherichia coli

Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells

Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells

Chapter 18. Protein Interaction Technologies





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